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adeno-associated virus encoding excitatory designer receptors exclusively activated by designer drugs (dreadds; aav8-hsyn-dio-hm3d(gq)-mcherry  (Addgene inc)


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    Addgene inc adeno-associated virus encoding excitatory designer receptors exclusively activated by designer drugs (dreadds; aav8-hsyn-dio-hm3d(gq)-mcherry
    Adeno Associated Virus Encoding Excitatory Designer Receptors Exclusively Activated By Designer Drugs (Dreadds; Aav8 Hsyn Dio Hm3d(gq) Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/designer+drugs+dreadd/aav8+hsyn+dio+mcherry/pmc10104524__pnas__2218142120__sapp-22-0-14
    Average 90 stars, based on 1 article reviews
    adeno-associated virus encoding excitatory designer receptors exclusively activated by designer drugs (dreadds; aav8-hsyn-dio-hm3d(gq)-mcherry - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Control:

    Article Title: A paraventricular thalamus to insular cortex glutamatergic projection gates "emotional" stress-induced binge eating in females.
    Article Snippet: .. Chemogenetic surgeries: either Cre-dependent inhibitory designer receptors exclusively activated by designer drugs (DREADD) AAV2-hSynDIO-hM4D(Gi)-mCherry (110 nL; titer ~5 × 1012/100 μL; Addgene) or control virus AAV1/2-CAG-FLEX-tdTomato (tdT, 110 nL; ~8.7 × 1011 vg/ml; prepared by S. Layfield and R. Bathgate, Florey) were stereotaxically injected bilaterally into PVT (n= 40) at 100 nL/min rate (Nanoject III) plus 5 min. PVT co-ordinates: AP −1.59mm, ML+ /−1.0 mm, DV −2.96mm, 20° angle (adapted from [26]). ..

    Article Title: A paraventricular thalamus to insular cortex glutamatergic projection gates “emotional” stress-induced binge eating in females
    Article Snippet: .. Chemogenetic surgeries: either Cre-dependent inhibitory designer receptors exclusively activated by designer drugs (DREADD) AAV2-hSyn-DIO-hM4D(Gi)-mCherry (110 nL; titer ~5 × 10 12 /100 μL; Addgene) or control virus AAV1/2-CAG-FLEX-tdTomato (tdT, 110 nL; ~8.7 × 10 11 vg/ml; prepared by S. Layfield and R. Bathgate, Florey) were stereotaxically injected bilaterally into PVT ( n = 40) at 100 nL/min rate (Nanoject III) plus 5 min. PVT co-ordinates: AP −1.59 mm, ML + /−1.0 mm, DV −2.96 mm, 20° angle (adapted from [ ]). ..

    Article Title: Nigrostriatal dopamine pathway regulates auditory discrimination behavior
    Article Snippet: .. The following viruses were used: retrograde axonal tracing from the striatum or retrograde optogenetic/imaging experiments: CAV2-Cre and CAV2-GFP (PVM, France); optogenetic silencing: AAV2/9-CAG-DIO-ArchT-GFP (UNC vector core) and AAV2/9-CAG-EGFP (Addgene, #37825) as experimental and control viruses, respectively; ex vivo optogenetic activation: AAV2/5-CAG-hChR2(H134R)-mCherry (Addgene, #100054); dopamine sensor imaging: AAV2/9-CAG-DA2m (WZ Bioscience, US; Yulong Li lab) and AAV2/9-CAG-EGFP (Addgene, #37825) as experimental and control viruses, respectively; calcium sensor microendoscopic imaging: AAV2/9-CAG-GCaMP6f (Addgene, # 100836) and AAV2/1-hSyn-DIO-GCaMP6f (Addgene, #100837); designer receptor exclusively activated by designer drugs (DREADD)-based chemogenetic silencing: AAV2/8-hSyn-DIO-M4Di-mCherry (Addgene, #44362) and AAV2/8-hSyn-DIO-mCherry (Addgene, # 50459) as experimental and control viruses, respectively; anterograde tracing from the SNc: AAV2/9-WGA-Cre (custom-made) and AAV2/8-DIO-tdTomato (Addgene, #28306). ..

    Virus:

    Article Title: A paraventricular thalamus to insular cortex glutamatergic projection gates "emotional" stress-induced binge eating in females.
    Article Snippet: .. Chemogenetic surgeries: either Cre-dependent inhibitory designer receptors exclusively activated by designer drugs (DREADD) AAV2-hSynDIO-hM4D(Gi)-mCherry (110 nL; titer ~5 × 1012/100 μL; Addgene) or control virus AAV1/2-CAG-FLEX-tdTomato (tdT, 110 nL; ~8.7 × 1011 vg/ml; prepared by S. Layfield and R. Bathgate, Florey) were stereotaxically injected bilaterally into PVT (n= 40) at 100 nL/min rate (Nanoject III) plus 5 min. PVT co-ordinates: AP −1.59mm, ML+ /−1.0 mm, DV −2.96mm, 20° angle (adapted from [26]). ..

    Article Title: A paraventricular thalamus to insular cortex glutamatergic projection gates “emotional” stress-induced binge eating in females
    Article Snippet: .. Chemogenetic surgeries: either Cre-dependent inhibitory designer receptors exclusively activated by designer drugs (DREADD) AAV2-hSyn-DIO-hM4D(Gi)-mCherry (110 nL; titer ~5 × 10 12 /100 μL; Addgene) or control virus AAV1/2-CAG-FLEX-tdTomato (tdT, 110 nL; ~8.7 × 10 11 vg/ml; prepared by S. Layfield and R. Bathgate, Florey) were stereotaxically injected bilaterally into PVT ( n = 40) at 100 nL/min rate (Nanoject III) plus 5 min. PVT co-ordinates: AP −1.59 mm, ML + /−1.0 mm, DV −2.96 mm, 20° angle (adapted from [ ]). ..

    Injection:

    Article Title: A paraventricular thalamus to insular cortex glutamatergic projection gates "emotional" stress-induced binge eating in females.
    Article Snippet: .. Chemogenetic surgeries: either Cre-dependent inhibitory designer receptors exclusively activated by designer drugs (DREADD) AAV2-hSynDIO-hM4D(Gi)-mCherry (110 nL; titer ~5 × 1012/100 μL; Addgene) or control virus AAV1/2-CAG-FLEX-tdTomato (tdT, 110 nL; ~8.7 × 1011 vg/ml; prepared by S. Layfield and R. Bathgate, Florey) were stereotaxically injected bilaterally into PVT (n= 40) at 100 nL/min rate (Nanoject III) plus 5 min. PVT co-ordinates: AP −1.59mm, ML+ /−1.0 mm, DV −2.96mm, 20° angle (adapted from [26]). ..

    Article Title: A paraventricular thalamus to insular cortex glutamatergic projection gates “emotional” stress-induced binge eating in females
    Article Snippet: .. Chemogenetic surgeries: either Cre-dependent inhibitory designer receptors exclusively activated by designer drugs (DREADD) AAV2-hSyn-DIO-hM4D(Gi)-mCherry (110 nL; titer ~5 × 10 12 /100 μL; Addgene) or control virus AAV1/2-CAG-FLEX-tdTomato (tdT, 110 nL; ~8.7 × 10 11 vg/ml; prepared by S. Layfield and R. Bathgate, Florey) were stereotaxically injected bilaterally into PVT ( n = 40) at 100 nL/min rate (Nanoject III) plus 5 min. PVT co-ordinates: AP −1.59 mm, ML + /−1.0 mm, DV −2.96 mm, 20° angle (adapted from [ ]). ..

    Article Title: Sexually dimorphic neural encoding of threat discrimination in nucleus accumbens afferents drives suppression of reward behavior
    Article Snippet: .. To achieve projection specific expression of inhibitory designer receptors exclusively activated by designer drugs (DREADD) for chemogenetic manipulation, 0.5 μl of AAV-rg-pkg-cre (1.17 × 10^13 GC/ml; Addgene); diluted with sterile PBS at a ratio of 1:4 was bilaterally injected into the NAc (AP:+1.30, ML: +/-1.81, D/V: −4.43, 15° angle) and 0.5 μl of AAV5-hSyn-DIO-hM4D(Gi)-mCherry (2.4 × 10^13 GC/ml; Addgene) or AAV5-hSyn-DIO-mCherry (2.3 × 10^13 GC/ml; Addgene) was bilaterally injected into either the mPFC (AP:1.7, ML: +/-0.75, D/V: −2.5, 12° angle) or vHip (AP:-3.40, ML: +/-3.95, D/V: −4.17, 12° angle). .. AAV-pgk-Cre was a gift from Patrick Aebischer (Addgene plasmid # 24593; http://n2t.net/addgene:24593 ; RRID:Addgene_24593). pAAV-hSyn-DIO-hM4D(Gi)-mCherry was a gift from Bryan Roth (Addgene plasmid # 44362; http://n2t.net/addgene:44362 ; RRID:Addgene_44362). pAAV-hSyn-DIO-mCherry was a gift from Bryan Roth (Addgene plasmid # 50459 ; http://n2t.net/addgene:50459 ; RRID:Addgene_50459).

    Expressing:

    Article Title: Sexually dimorphic neural encoding of threat discrimination in nucleus accumbens afferents drives suppression of reward behavior
    Article Snippet: .. To achieve projection specific expression of inhibitory designer receptors exclusively activated by designer drugs (DREADD) for chemogenetic manipulation, 0.5 μl of AAV-rg-pkg-cre (1.17 × 10^13 GC/ml; Addgene); diluted with sterile PBS at a ratio of 1:4 was bilaterally injected into the NAc (AP:+1.30, ML: +/-1.81, D/V: −4.43, 15° angle) and 0.5 μl of AAV5-hSyn-DIO-hM4D(Gi)-mCherry (2.4 × 10^13 GC/ml; Addgene) or AAV5-hSyn-DIO-mCherry (2.3 × 10^13 GC/ml; Addgene) was bilaterally injected into either the mPFC (AP:1.7, ML: +/-0.75, D/V: −2.5, 12° angle) or vHip (AP:-3.40, ML: +/-3.95, D/V: −4.17, 12° angle). .. AAV-pgk-Cre was a gift from Patrick Aebischer (Addgene plasmid # 24593; http://n2t.net/addgene:24593 ; RRID:Addgene_24593). pAAV-hSyn-DIO-hM4D(Gi)-mCherry was a gift from Bryan Roth (Addgene plasmid # 44362; http://n2t.net/addgene:44362 ; RRID:Addgene_44362). pAAV-hSyn-DIO-mCherry was a gift from Bryan Roth (Addgene plasmid # 50459 ; http://n2t.net/addgene:50459 ; RRID:Addgene_50459).

    Bioprocessing:

    Article Title: Sexually dimorphic neural encoding of threat discrimination in nucleus accumbens afferents drives suppression of reward behavior
    Article Snippet: .. To achieve projection specific expression of inhibitory designer receptors exclusively activated by designer drugs (DREADD) for chemogenetic manipulation, 0.5 μl of AAV-rg-pkg-cre (1.17 × 10^13 GC/ml; Addgene); diluted with sterile PBS at a ratio of 1:4 was bilaterally injected into the NAc (AP:+1.30, ML: +/-1.81, D/V: −4.43, 15° angle) and 0.5 μl of AAV5-hSyn-DIO-hM4D(Gi)-mCherry (2.4 × 10^13 GC/ml; Addgene) or AAV5-hSyn-DIO-mCherry (2.3 × 10^13 GC/ml; Addgene) was bilaterally injected into either the mPFC (AP:1.7, ML: +/-0.75, D/V: −2.5, 12° angle) or vHip (AP:-3.40, ML: +/-3.95, D/V: −4.17, 12° angle). .. AAV-pgk-Cre was a gift from Patrick Aebischer (Addgene plasmid # 24593; http://n2t.net/addgene:24593 ; RRID:Addgene_24593). pAAV-hSyn-DIO-hM4D(Gi)-mCherry was a gift from Bryan Roth (Addgene plasmid # 44362; http://n2t.net/addgene:44362 ; RRID:Addgene_44362). pAAV-hSyn-DIO-mCherry was a gift from Bryan Roth (Addgene plasmid # 50459 ; http://n2t.net/addgene:50459 ; RRID:Addgene_50459).

    Sterility:

    Article Title: Sexually dimorphic neural encoding of threat discrimination in nucleus accumbens afferents drives suppression of reward behavior
    Article Snippet: .. To achieve projection specific expression of inhibitory designer receptors exclusively activated by designer drugs (DREADD) for chemogenetic manipulation, 0.5 μl of AAV-rg-pkg-cre (1.17 × 10^13 GC/ml; Addgene); diluted with sterile PBS at a ratio of 1:4 was bilaterally injected into the NAc (AP:+1.30, ML: +/-1.81, D/V: −4.43, 15° angle) and 0.5 μl of AAV5-hSyn-DIO-hM4D(Gi)-mCherry (2.4 × 10^13 GC/ml; Addgene) or AAV5-hSyn-DIO-mCherry (2.3 × 10^13 GC/ml; Addgene) was bilaterally injected into either the mPFC (AP:1.7, ML: +/-0.75, D/V: −2.5, 12° angle) or vHip (AP:-3.40, ML: +/-3.95, D/V: −4.17, 12° angle). .. AAV-pgk-Cre was a gift from Patrick Aebischer (Addgene plasmid # 24593; http://n2t.net/addgene:24593 ; RRID:Addgene_24593). pAAV-hSyn-DIO-hM4D(Gi)-mCherry was a gift from Bryan Roth (Addgene plasmid # 44362; http://n2t.net/addgene:44362 ; RRID:Addgene_44362). pAAV-hSyn-DIO-mCherry was a gift from Bryan Roth (Addgene plasmid # 50459 ; http://n2t.net/addgene:50459 ; RRID:Addgene_50459).

    Plasmid Preparation:

    Article Title: A Critical Involvement of Glutamatergic Neurons in the Anterior Insular Cortex for Subdiaphragmatic Vagotomy-induced Analgesia.
    Article Snippet: .. The following vector was used for single-site designer receptors exclusively activated by designer drugs (DREADD) pAAVCaMKIIa-hM3D(Gq)-mCherry (50476, Addgene, MA, USA). ..

    Ex Vivo:

    Article Title: Nigrostriatal dopamine pathway regulates auditory discrimination behavior
    Article Snippet: .. The following viruses were used: retrograde axonal tracing from the striatum or retrograde optogenetic/imaging experiments: CAV2-Cre and CAV2-GFP (PVM, France); optogenetic silencing: AAV2/9-CAG-DIO-ArchT-GFP (UNC vector core) and AAV2/9-CAG-EGFP (Addgene, #37825) as experimental and control viruses, respectively; ex vivo optogenetic activation: AAV2/5-CAG-hChR2(H134R)-mCherry (Addgene, #100054); dopamine sensor imaging: AAV2/9-CAG-DA2m (WZ Bioscience, US; Yulong Li lab) and AAV2/9-CAG-EGFP (Addgene, #37825) as experimental and control viruses, respectively; calcium sensor microendoscopic imaging: AAV2/9-CAG-GCaMP6f (Addgene, # 100836) and AAV2/1-hSyn-DIO-GCaMP6f (Addgene, #100837); designer receptor exclusively activated by designer drugs (DREADD)-based chemogenetic silencing: AAV2/8-hSyn-DIO-M4Di-mCherry (Addgene, #44362) and AAV2/8-hSyn-DIO-mCherry (Addgene, # 50459) as experimental and control viruses, respectively; anterograde tracing from the SNc: AAV2/9-WGA-Cre (custom-made) and AAV2/8-DIO-tdTomato (Addgene, #28306). ..

    Activation Assay:

    Article Title: Nigrostriatal dopamine pathway regulates auditory discrimination behavior
    Article Snippet: .. The following viruses were used: retrograde axonal tracing from the striatum or retrograde optogenetic/imaging experiments: CAV2-Cre and CAV2-GFP (PVM, France); optogenetic silencing: AAV2/9-CAG-DIO-ArchT-GFP (UNC vector core) and AAV2/9-CAG-EGFP (Addgene, #37825) as experimental and control viruses, respectively; ex vivo optogenetic activation: AAV2/5-CAG-hChR2(H134R)-mCherry (Addgene, #100054); dopamine sensor imaging: AAV2/9-CAG-DA2m (WZ Bioscience, US; Yulong Li lab) and AAV2/9-CAG-EGFP (Addgene, #37825) as experimental and control viruses, respectively; calcium sensor microendoscopic imaging: AAV2/9-CAG-GCaMP6f (Addgene, # 100836) and AAV2/1-hSyn-DIO-GCaMP6f (Addgene, #100837); designer receptor exclusively activated by designer drugs (DREADD)-based chemogenetic silencing: AAV2/8-hSyn-DIO-M4Di-mCherry (Addgene, #44362) and AAV2/8-hSyn-DIO-mCherry (Addgene, # 50459) as experimental and control viruses, respectively; anterograde tracing from the SNc: AAV2/9-WGA-Cre (custom-made) and AAV2/8-DIO-tdTomato (Addgene, #28306). ..

    Imaging:

    Article Title: Nigrostriatal dopamine pathway regulates auditory discrimination behavior
    Article Snippet: .. The following viruses were used: retrograde axonal tracing from the striatum or retrograde optogenetic/imaging experiments: CAV2-Cre and CAV2-GFP (PVM, France); optogenetic silencing: AAV2/9-CAG-DIO-ArchT-GFP (UNC vector core) and AAV2/9-CAG-EGFP (Addgene, #37825) as experimental and control viruses, respectively; ex vivo optogenetic activation: AAV2/5-CAG-hChR2(H134R)-mCherry (Addgene, #100054); dopamine sensor imaging: AAV2/9-CAG-DA2m (WZ Bioscience, US; Yulong Li lab) and AAV2/9-CAG-EGFP (Addgene, #37825) as experimental and control viruses, respectively; calcium sensor microendoscopic imaging: AAV2/9-CAG-GCaMP6f (Addgene, # 100836) and AAV2/1-hSyn-DIO-GCaMP6f (Addgene, #100837); designer receptor exclusively activated by designer drugs (DREADD)-based chemogenetic silencing: AAV2/8-hSyn-DIO-M4Di-mCherry (Addgene, #44362) and AAV2/8-hSyn-DIO-mCherry (Addgene, # 50459) as experimental and control viruses, respectively; anterograde tracing from the SNc: AAV2/9-WGA-Cre (custom-made) and AAV2/8-DIO-tdTomato (Addgene, #28306). ..

    Anterograde Tracing:

    Article Title: Nigrostriatal dopamine pathway regulates auditory discrimination behavior
    Article Snippet: .. The following viruses were used: retrograde axonal tracing from the striatum or retrograde optogenetic/imaging experiments: CAV2-Cre and CAV2-GFP (PVM, France); optogenetic silencing: AAV2/9-CAG-DIO-ArchT-GFP (UNC vector core) and AAV2/9-CAG-EGFP (Addgene, #37825) as experimental and control viruses, respectively; ex vivo optogenetic activation: AAV2/5-CAG-hChR2(H134R)-mCherry (Addgene, #100054); dopamine sensor imaging: AAV2/9-CAG-DA2m (WZ Bioscience, US; Yulong Li lab) and AAV2/9-CAG-EGFP (Addgene, #37825) as experimental and control viruses, respectively; calcium sensor microendoscopic imaging: AAV2/9-CAG-GCaMP6f (Addgene, # 100836) and AAV2/1-hSyn-DIO-GCaMP6f (Addgene, #100837); designer receptor exclusively activated by designer drugs (DREADD)-based chemogenetic silencing: AAV2/8-hSyn-DIO-M4Di-mCherry (Addgene, #44362) and AAV2/8-hSyn-DIO-mCherry (Addgene, # 50459) as experimental and control viruses, respectively; anterograde tracing from the SNc: AAV2/9-WGA-Cre (custom-made) and AAV2/8-DIO-tdTomato (Addgene, #28306). ..



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